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<br /> BioProcess<br /> International<br /> DIGITAL EDITION<br /> ARCHIVE<br /> SPECIAL REPORT<br /> Accelerating the<br /> Development and<br /> Manufacture<br /> of Therapeutics<br /> Using the<br /> Octet Platform<br /> Accelerating the Development<br /> and Manufacture of Therapeutics<br /> Using the Octet Platform<br /> David Apiyo<br /> I<br /> dentifying a promising biotherapeutic candidate,<br /> developing it for a preclinical program, and<br /> designing a robust manufacturing process all<br /> require complex, multistep workflows. The steps<br /> include the determination of titers and cell-line<br /> expression levels, assessment of the kinetics of<br /> target molecule binding, determination of a<br /> therapeutic's potency, and confirmation of critical<br /> quality attributes (CQAs). Traditional analytical<br /> techniques that measure protein quality and<br /> quantity include UV spectroscopy, enzyme-linked<br /> immu<a title="September 2021 - Special Report - Sartorius Octet page 1" href="https://secure.viewer.zmags.com/publication/745270a6?page=1"> BioProcess International DIGITAL EDITION ARCHIVE</a> <a title="September 2021 - Special Report - Sartorius Octet page 2" href="https://secure.viewer.zmags.com/publication/745270a6?page=2"> SPECIAL REPORT A</a> <a title="September 2021 - Special Report - Sartorius Octet page 3" href="https://secure.viewer.zmags.com/publication/745270a6?page=3"> Accelerating the Development and Manufacture of T</a> <a title="September 2021 - Special Report - Sartorius Octet page 4" href="https://secure.viewer.zmags.com/publication/745270a6?page=4"> Figure 2: Applications of biolayer interferometry</a> <a title="September 2021 - Special Report - Sartorius Octet page 5" href="https://secure.viewer.zmags.com/publication/745270a6?page=5"> Table 1: Quantitation assay formats and features </a> <a title="September 2021 - Special Report - Sartorius Octet page 6" href="https://secure.viewer.zmags.com/publication/745270a6?page=6"> Figure 4: Comparing protein quantitation in compl</a> <a title="September 2021 - Special Report - Sartorius Octet page 7" href="https://secure.viewer.zmags.com/publication/745270a6?page=7"> Figure 6: Protein titer assessment and growth med</a> <a title="September 2021 - Special Report - Sartorius Octet page 8" href="https://secure.viewer.zmags.com/publication/745270a6?page=8"> Figure 7: The Octet platform can be used througho</a> <a title="September 2021 - Special Report - Sartorius Octet page 9" href="https://secure.viewer.zmags.com/publication/745270a6?page=9"> Figure 10: High-performance liquid chromatography</a> <a title="September 2021 - Special Report - Sartorius Octet page 10" href="https://secure.viewer.zmags.com/publication/745270a6?page=10"> Figure 11: The Octet binding kinetics and functio</a> <a title="September 2021 - Special Report - Sartorius Octet page 11" href="https://secure.viewer.zmags.com/publication/745270a6?page=11"> Figure 14: Comparing process steps and time requi</a> <a title="September 2021 - Special Report - Sartorius Octet page 12" href="https://secure.viewer.zmags.com/publication/745270a6?page=12"> vaccine (14). The polyclonal antibody is bound to</a> <a title="September 2021 - Special Report - Sartorius Octet page 13" href="https://secure.viewer.zmags.com/publication/745270a6?page=13"> Label Free. Stress Free. Introducing the Octet® R</a>