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<br /> BioProcess<br /> International<br /> DIGITAL EDITION<br /> ARCHIVE<br /> March 2018 | Volume 16 | Number 3<br /> www.bioprocessintl.com<br /> COVERING THE WHOLE DEVELOPMENT PROCESS FOR THE GLOBAL BIOTECHNOLOGY INDUSTRY<br /> DOWNSTREAM/MANUFACTURING<br /> Communicating Quality<br /> Membrane HIC for MAbs<br /> IgG Ultrafiltration<br /> Enhancing Galactosylation<br /> Affinity for Blood Factors<br /> Vendors and Viral Safety<br /> From DNA to 1–100 g of protein in 6 WEEKS!<br /> the next<br /> medicine...<br /> We'll develop it together.<br /> As a leader for contract development and We manage projects from pre-clinical stage<br /> As a leader for contract development and We manage projects from pre-clinical stage<br /> manufacturing, we at Lonza Pharma & Biotech through to commercialization, and our expertise<br /> manufacturing, we at Lonza Pharma & Biotech through to commercializat<a title="March 2018 page 1" href="https://secure.viewer.zmags.com/publication/944ca7af?page=1"> BioProcess International DIGITAL EDITION ARCHIVE</a> <a title="March 2018 page 2" href="https://secure.viewer.zmags.com/publication/944ca7af?page=2"> March 2018 | Volume 16 | Number 3 www.bioprocessi</a> <a title="March 2018 page 3" href="https://secure.viewer.zmags.com/publication/944ca7af?page=3"> From DNA to 1–100 g of protein in 6 WEEKS!</a> <a title="March 2018 page 4" href="https://secure.viewer.zmags.com/publication/944ca7af?page=4"> the next medicine... We'll develop it together. </a> <a title="March 2018 page 5" href="https://secure.viewer.zmags.com/publication/944ca7af?page=5"> VOLUME 16 NUMBER 3 ON THE COVER Downstream suit</a> <a title="March 2018 page 6" href="https://secure.viewer.zmags.com/publication/944ca7af?page=6"> Intensify your downstream process Unlock process</a> <a title="March 2018 page 7" href="https://secure.viewer.zmags.com/publication/944ca7af?page=7"> www.bioprocessintl.com/eBooks/ Our 2018 eBooks a</a> <a title="March 2018 page 8" href="https://secure.viewer.zmags.com/publication/944ca7af?page=8"> THe 6 sHarpest perspectives for focused science a</a> <a title="March 2018 page 9" href="https://secure.viewer.zmags.com/publication/944ca7af?page=9"> FROM THE EDITOR A re you prote</a> <a title="March 2018 page 10" href="https://secure.viewer.zmags.com/publication/944ca7af?page=10"> 6 powerful propellers to accelerate expert scienc</a> <a title="March 2018 page 11" href="https://secure.viewer.zmags.com/publication/944ca7af?page=11"> Welcome New Editorial Advisor Kavita Ramalingam </a> <a title="March 2018 page 12" href="https://secure.viewer.zmags.com/publication/944ca7af?page=12"> Bioproduction Access to the best manufact</a> <a title="March 2018 page 13" href="https://secure.viewer.zmags.com/publication/944ca7af?page=13"> Organization: “The pace of scientific pro</a> <a title="March 2018 page 14" href="https://secure.viewer.zmags.com/publication/944ca7af?page=14"> With Pfizer CentreOne contract manufacturing, you </a> <a title="March 2018 page 15" href="https://secure.viewer.zmags.com/publication/944ca7af?page=15"> of clinical and commercial needs,” says John Chim</a> <a title="March 2018 page 16" href="https://secure.viewer.zmags.com/publication/944ca7af?page=16"> In 2017, CGTC acquired Catapult Therapy TCR</a> <a title="March 2018 page 17" href="https://secure.viewer.zmags.com/publication/944ca7af?page=17"> FOCUS ON... BUSINESS Back to Basics for </a> <a title="March 2018 page 18" href="https://secure.viewer.zmags.com/publication/944ca7af?page=18"> Consistency. Proven CHO cells are one of </a> <a title="March 2018 page 19" href="https://secure.viewer.zmags.com/publication/944ca7af?page=19"> CONNECT THE DOTS FOR EMPLOYEES It's clear that ha</a> <a title="March 2018 page 20" href="https://secure.viewer.zmags.com/publication/944ca7af?page=20"> Passion for Performance </a> <a title="March 2018 page 21" href="https://secure.viewer.zmags.com/publication/944ca7af?page=21"> Such practical recognition activities, when </a> <a title="March 2018 page 22" href="https://secure.viewer.zmags.com/publication/944ca7af?page=22"> accountable for communicating with their teams an</a> <a title="March 2018 page 23" href="https://secure.viewer.zmags.com/publication/944ca7af?page=23"> BIOPROCESS TECHNICAL A Two-Step Purification Pr</a> <a title="March 2018 page 24" href="https://secure.viewer.zmags.com/publication/944ca7af?page=24"> Superior Binding Capacity High Purity Excelle</a> <a title="March 2018 page 25" href="https://secure.viewer.zmags.com/publication/944ca7af?page=25"> Figure 2: Full-factorial DoE (molecule 2) with t</a> <a title="March 2018 page 26" href="https://secure.viewer.zmags.com/publication/944ca7af?page=26"> CATEGORIES DEVELOPMENT RELIABILITY EXPERTI</a> <a title="March 2018 page 27" href="https://secure.viewer.zmags.com/publication/944ca7af?page=27"> Table 1: Step performance summary for molecule 1</a> <a title="March 2018 page 28" href="https://secure.viewer.zmags.com/publication/944ca7af?page=28"> Figure 4: 170 160 150 140 130 120 110 100 90 80 </a> <a title="March 2018 page 29" href="https://secure.viewer.zmags.com/publication/944ca7af?page=29"> BIOPROCESS TECHNICAL IgG Purification By Ultraf</a> <a title="March 2018 page 30" href="https://secure.viewer.zmags.com/publication/944ca7af?page=30"> We Meet Your Single Use Process Monitoring </a> <a title="March 2018 page 31" href="https://secure.viewer.zmags.com/publication/944ca7af?page=31"> Figure 2: Dissociation of contaminant subsets co</a> <a title="March 2018 page 32" href="https://secure.viewer.zmags.com/publication/944ca7af?page=32"> A 10% improvement in yield!?! Sent 9:51 AM Ye</a> <a title="March 2018 page 33" href="https://secure.viewer.zmags.com/publication/944ca7af?page=33"> Figure 4: Flow diagrams through an apparatus con</a> <a title="March 2018 page 34" href="https://secure.viewer.zmags.com/publication/944ca7af?page=34"> Driving innovation since 1981 Security of supp</a> <a title="March 2018 page 35" href="https://secure.viewer.zmags.com/publication/944ca7af?page=35"> Figure 6: Efficiency of media use by different c</a> <a title="March 2018 page 36" href="https://secure.viewer.zmags.com/publication/944ca7af?page=36"> CONVERT YOUR EXISTIN</a> <a title="March 2018 page 37" href="https://secure.viewer.zmags.com/publication/944ca7af?page=37"> Figure 7: Experimental results from foulant remo</a> <a title="March 2018 page 38" href="https://secure.viewer.zmags.com/publication/944ca7af?page=38"> Figure 9: Experimental size-exclusion chromatogr</a> <a title="March 2018 page 39" href="https://secure.viewer.zmags.com/publication/944ca7af?page=39"> BIOPROCESS TECHNICAL Enhanced Galactosylation o</a> <a title="March 2018 page 40" href="https://secure.viewer.zmags.com/publication/944ca7af?page=40"> ARE YOU LOOKING FOR EXPERTS IN MICROBIAL PRODUC</a> <a title="March 2018 page 41" href="https://secure.viewer.zmags.com/publication/944ca7af?page=41"> Figure 1: Effects of uridine–Mn–galactose mixtur</a> <a title="March 2018 page 42" href="https://secure.viewer.zmags.com/publication/944ca7af?page=42"> HIS IS SINGLE-USE REINVENTED SciLog® SE</a> <a title="March 2018 page 43" href="https://secure.viewer.zmags.com/publication/944ca7af?page=43"> enzyme. In addition, galactosylation stab</a> <a title="March 2018 page 44" href="https://secure.viewer.zmags.com/publication/944ca7af?page=44"> April 24th 2018 Radisson BLU S</a> <a title="March 2018 page 45" href="https://secure.viewer.zmags.com/publication/944ca7af?page=45"> observed during studies using the branched trisac</a> <a title="March 2018 page 46" href="https://secure.viewer.zmags.com/publication/944ca7af?page=46"> 25 Wormald MR, et al. Variations in Oligos</a> <a title="March 2018 page 47" href="https://secure.viewer.zmags.com/publication/944ca7af?page=47"> BIOPROCESS TECHNICAL Large-Scale Purification o</a> <a title="March 2018 page 48" href="https://secure.viewer.zmags.com/publication/944ca7af?page=48"> Our column range has evolved! </a> <a title="March 2018 page 49" href="https://secure.viewer.zmags.com/publication/944ca7af?page=49"> heparin, murine antihuman F-IX antibodies, metal </a> <a title="March 2018 page 50" href="https://secure.viewer.zmags.com/publication/944ca7af?page=50"> X When pu</a> <a title="March 2018 page 51" href="https://secure.viewer.zmags.com/publication/944ca7af?page=51"> Figure 3: 10% silver-stained sodium-dodecyl sulf</a> <a title="March 2018 page 52" href="https://secure.viewer.zmags.com/publication/944ca7af?page=52"> DRIVING THE FUTURE OF THE LIFE SCIENCE INDUSTRY #</a> <a title="March 2018 page 53" href="https://secure.viewer.zmags.com/publication/944ca7af?page=53"> GE Healthcare) and virus filtered the eluate with</a> <a title="March 2018 page 54" href="https://secure.viewer.zmags.com/publication/944ca7af?page=54"> insignif icant amount of impurities as measured b</a> <a title="March 2018 page 55" href="https://secure.viewer.zmags.com/publication/944ca7af?page=55"> SUPPLIER Side Advanced Viral Clearance Study Des</a> <a title="March 2018 page 56" href="https://secure.viewer.zmags.com/publication/944ca7af?page=56"> produced with typical purification strategies (no</a> <a title="March 2018 page 57" href="https://secure.viewer.zmags.com/publication/944ca7af?page=57"> MVM virus stocks. QC analysis of the ultrapurifie</a> <a title="March 2018 page 58" href="https://secure.viewer.zmags.com/publication/944ca7af?page=58"> Premier Association </a> <a title="March 2018 page 59" href="https://secure.viewer.zmags.com/publication/944ca7af?page=59"> Table 3: Viral clearance data resulting from fil</a> <a title="March 2018 page 60" href="https://secure.viewer.zmags.com/publication/944ca7af?page=60"> manufacturing is complicated by variations in pot</a> <a title="March 2018 page 61" href="https://secure.viewer.zmags.com/publication/944ca7af?page=61"> SUPPLIER Side Evaluating Adsorptive Filtration A</a> <a title="March 2018 page 62" href="https://secure.viewer.zmags.com/publication/944ca7af?page=62"> used ÄKTAexplorer 10 systems with Unicorn softwar</a> <a title="March 2018 page 63" href="https://secure.viewer.zmags.com/publication/944ca7af?page=63"> Figure 1: Log reduction values (LRVs) for (A) </a> <a title="March 2018 page 64" href="https://secure.viewer.zmags.com/publication/944ca7af?page=64"> Figure 3: Log reduction values (LRVs) for 10 X</a> <a title="March 2018 page 65" href="https://secure.viewer.zmags.com/publication/944ca7af?page=65"> determined LRVs. Current results showed only mino</a> <a title="March 2018 page 66" href="https://secure.viewer.zmags.com/publication/944ca7af?page=66"> Continued from page 35 removal and ultrafiltratio</a> <a title="March 2018 page 67" href="https://secure.viewer.zmags.com/publication/944ca7af?page=67"> %% Reverse Translational Medicine The Promising</a> <a title="March 2018 page 68" href="https://secure.viewer.zmags.com/publication/944ca7af?page=68"> BIO 2018 Registration is Now Open! The BIO In</a> <a title="March 2018 page 69" href="https://secure.viewer.zmags.com/publication/944ca7af?page=69"> smart biologics. biologics single partner. new! t</a>